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［摘
要］ 目的
探讨IL-1（IL1α，IL1β）对长波紫外线（UVA）辐射后成纤维细胞基质金属蛋白酶（matrix
metalloproteinases，MMPs）表达的影响机制。
方法
用ELISA法检测UVA辐射后成纤维细胞培养上清MMP-1和MMP-2的表达。接着用IL-1α和IL-1β分别处理UVA辐射后的成纤维细胞，用Western免疫印迹法检测其丝裂原活化蛋白激酶（MAPK）的活性；用RT-PCR方法检测c-fos
和c-jun的mRNA表达。
结果
不同剂量UVA（0，1，5，10
J/cm2）辐射的成纤维细胞分泌MMP-1逐渐上升，对MMP-2分泌没有影响。IL-1α和IL-1β（0，1，10，100
ng/ml）促进UVA（10 J/cm2）辐射成纤维细胞的MAPK活性表达，并以剂量依赖方式促进c-jun的mRNA表达。IL-1α还显著增加c-fos
mRNA表达，但IL-1β对c-fos
mRNA表达无明显影响。IL-1α和IL-1β促进UVA辐射成纤维细胞分泌MMP-1，于100
ng/ml时有显著性差异（P均<0.05），但对MMP-2分泌无明显影响。
结论
UVA辐射成纤维细胞分泌MMP-1增加，对MMP-2分泌没有影响。IL-1（IL-1α和IL-1β）通过促进MAPK活性和c-jun
mRNA表达，IL-1α还促进cfos
mRNA表达使UVA辐射成纤维细胞MMP-1表达增加，表明IL-1在皮肤光老化的真皮胶原过度降解中发挥着重要的作用。
［关键词］IL-1α；IL-1β；基质金属蛋白酶；成纤维细胞
［中图分类号］
R 711.72 ［文献标识码］ A ［文章编号］
1001-7089(2005)09-0-0
Study
the Effects Mechanism of IL1 on MMP
Production of UVAirradiated Fibroblasts
WANG
Xiaoyong，BI Zhigang
(Department of Dermatology，the First
Affiliated Hospital of Nanjing Medical University，Nanjing，210029，China)
Abstract:
Objective To explore the effects
mechanism of IL-1（IL-1α，IL-1β）on
matrix metalloproteinase(MMP) production of UVAirradiated
fibroblasts.
Methods
MMP-1 and MMP-2 production in UVAirradiated
fibroblasts culture medium was detected by ELISA. UVAirradiated
fibroblasts were treated with IL-1αor IL-1β
separately，which mitogenactivated
protein kinase(MAPK) activity expression was determined by Western
immunoblot，c-fos and c-jun mRNA was detected
by RT-PCR，MMP-1 and MMP-2 production was
detected by ELISA.
Results
Different dosage of UVA(0，1，5，10，15
mJ/cm2) increased gradually MMP-1 production
of fibroblasts but had no effects on MMP-2 production.IL1α
and IL-1β（0，1，10，100
ng/ml）dosedependently
promoted MAPK activity and cjun mRNA
expression of UVA（10 J/cm2）irradiated
fibroblasts.IL1α
significantly increased c-fos mRNA expression too，while
IL-1βhad no significantly effects on c-fos
mRNA expression.IL-1αand IL-1β
promoted MMP-1 production of UVA （10 J/cm2）irradiated
fibroblasts while had no significantly effects on MMP-2 production，significant
difference was observed at dose 100 ng/ml (P<0.05).
Conclusion
UVA irradiation increases MMP-1 production of
fibroblasts but has no effects on MMP-2 production.IL-1（IL-1α，IL-1β）increases
MMP-1 production of UVAirradiated fibroblasts
through promoting MAPK activity and c-jun mRNA expression，IL-1αincreases
c-fos mRNA expression also.Our data indicate that IL-1 exerts important
effects on dermal collagen excessive degradation of skin photoaging.
Key
words： IL-1α;IL-1β;MMPs;Fibroblasts
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