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［摘
要］ 目的
研究甲氨蝶呤(methotrexate，
MTX)对银屑病皮损内血管内皮生长因子（VEGF）表达的影响，探讨其对银屑病的治疗机理。
方法
随机选择12例正常对照者和13例中度、重度银屑病患者，所有患者治疗前和治疗6周后行皮肤活检。利用巢式聚合酶链反应法测定12例正常对照和13例银屑病患者治疗前及治疗6周后皮损内VEGF
mRNA的定量。
结果
银屑病皮损内VEGF mRNA高于正常对照者皮肤内的水平（P＝0.005）。银屑病患者经过MTX治疗6周后，皮损明显改善，皮损内VEGF
mRNA的水平低于银屑病患者治疗前皮损内的水平（P＝0.030），但与正常对照者皮肤内的水平无统计学差异（P＝0.687）。
结论
MTX能抑制银屑病皮损内VEGF mRNA的过度表达，这可能是MTX治疗银屑病的作用机制之一。
［关键词］
甲氨蝶呤；银屑病；血管内皮生长因子；巢式聚合酶链反应
［中图分类号］
R 758.63 ［文献标识码］ A ［文章编号］
1001-7089(2005)09-0-0
Inhibition
Effects of Methotrexate on VEGF mRNA Expression in Psoriatic Lesional
Skin
LI
Changxing， ZHANG Xibao，WU
Zhihua，et al
(Department of Dermatology，Affilliated
Hospital of Guangdong Medical College，Zhanjiang
524001，China)
Abstract:
Objective To determine whether
methotrexate inhibition vascular endothelial growth factor (VEGF)mRNA
expression in psoriatic lesional skin.
Methods
Thirteen adult patients with moderate to severe
psoriasis and 12 health control were sequentially enrolled in this study，and
all patients' biopsies were taken before methotrexate treatment from
untreated lesions and after 6 weeks methotrexate treatment from
resolving lesions.The production VEGF mRNA was measured by nested
polymerase chain reaction.
Results
Expression of VEGF mRNA in psoriasis lesional skin
was higher than that in control subject (P＝0.005).Expression
of VEGF mRNA in psoriasis lesional skin was decreased after 6 weeks
methotrexate treatment (P＝0.030).Expression of
VEGF mRNA from psoriatic resolving lesions after treatment similar to
the control subject (P＝0.687).
Conclusion
Our results show that MTX could inhabit the over
expression of VEGF mRNA.These changes could be part of the therapeutic
effects of MTX on psoriasis.
Key
words： Methotrexate;Psoriasis;Vascular
endothelial growth factor;Nested polymerase chain reaction
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